Hello, I am a first time user of this toolbox for the purpose of unmixing some fluorescence images.
I have 2 8-bit tif stacks each of dimension [512, 512, 32] where 32 is the number of slices. They come from different channel wavelengths. I’d like to know how to use this toolbox to unmix each of these stacks (because it is believed that each channel has some infra signals that need to be separated).
In the documentation, the input image is said to have the dimension l*n where n is the number of channels. So, should i reshape the tif files as [512,512,32,2] ?
I tried to make a hyperstack using ImageJ with each of the image stacks above. Choosing 512 mb as RAM, i click ‘execute’ on the EndmemberNumberEstimation page of montevardi application. The end members estimates comes up as 1 with “virtual dimensionality” algorithm. Next, i select the VertexComponentAnalysis" and run it with the hyperstack. This gives the error: : Invalid Matrix size. Number of columns must be the same as the image number of channels.
Can someone please tell me how to use this app with the above image stacks ?
Platform: Windows 10, Orfeo toolbox 7.2.0